The central domain of Rhizobium leguminosarum DctD functions independently to activate transcription.
نویسندگان
چکیده
Sigma 54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal domains of R. leguminosarum DctD and have demonstrated that the central domain of DctD, like that of NifA, is transcriptionally competent.
منابع مشابه
The central domain of Rhizobium meliloti NifA is sufficient to activate transcription from the R. meliloti nifH promoter.
The Rhizobium meliloti nifA product (NifA) shares extensive homology in its central region and at its C-terminal end with Rhizobium leguminosarum DctD and with NtrC from several species. All three proteins are transcriptional activators of NtrA (RpoN)-RNA polymerase-dependent promoters. Several large deletions of R. meliloti NifA were constructed to investigate the role of the conserved and div...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 174 4 شماره
صفحات -
تاریخ انتشار 1992